DENUDATION KIT

DENUDATION KIT
IVF MEDIA

DK-640-1ML

DENUDATION KIT

Hyaluronidase 80 IU/ml

W/Gentamicin sulfate

W/Human serum albumin

W /HEPES buffered HTF


A. USAGE:

  • For cleans fresh oocytes prior to ICSI or other Assisted Reproductive Technologies (ART) Procedures.


B. CONTENTS:

  • Hyaluronidase solution (HS).
  • Product insert.


C. STORAGE:

  • The kit keeps dry at 2 – 8 °C.


D. STERILITY:

  • Sterile filtered.


E. COMPOSITION: 

Inorganic salts

  • Sodium chloride
  • Potassium chloride
  • Magnesium sulfate
  • Potassium phosphate
  • Calcium chloride

Buffers

  • Sodium bicarbonate
  • HEPES

Antibiotics

  • Gentamicin sulfate

Energy sources

  • Dextrose
  • Pyruvate
  • Lactate

Protein

  • Human serum albumin

Enzyme

  • Hyaluronidase

Indicator

  • Phenol red

Water

  • WFI quality


F. PRECAUTIONS:

  • Respect storage conditions of the product.
  • Do not use the product after its expiry date.
  • Do not use the product packaging appears damaged or if the seal is broken.
  • Store product in an area protected from light.
  • Manipulate the product in aseptic conditions (e.g.: under laminar air flow).
  • Wear clothes adapted to the manipulation of the product to avoid contamination (e.g.: gloves, mask, hygiene cap, overall, … etc.).
  • The product is intended to be used in vitro, in laboratory only.
  • Do not use it in therapy, human or veterinary applications.


G. PROCEDURES:

  • BioMEDIA provide easy denudation protocol for oocytes
  • Pre-equilibrate the Hyaluronidase Solution to 37 °C before use.
  • Dispense contents of the vial into an appropriate culture dish and place the oocytes in the Hyaluronidase solution for no more than 30 seconds while stripping off the surrounding cumulus cells with continuous pipetting.
  • Immediately remove the oocytes with a minimal volume of solution (leaving behind as much cumulus as possible) and transfer to wash medium.
  • Aspirate the oocytes with a pipette to remove any remaining cumulus and corona cells.
  • Wash the denuded oocytes 2 more times until completely denuded.
  • Denuded oocytes should be cultured for a minimum of 30 – 60 minutes before beginning ICSI procedures.

 

H. TECHNICAL ASPECTS:

  • Do not expose to temperatures greater than 37 °C.
  • Do not expose oocytes to the Hyaluronidase solution for greater than 30 seconds.
  • This buffering system provides optimum pH maintenance over the physiologic range (7.2 – 7.4) and does not require the use of a CO2 incubator.
  • Volume : 6 Vial × 1 ml
  • Storage : 2 – 8 °C
  • Cat. No. : DK-640-1ML
  • Shelf Life : 12 Months
  • Sterility : Sterile Filtered
  • State : Liquid

Download DENUDATION KIT Formulation.pdf

Download DENUDATION KIT Product Insert.pdf

Download DENUDATION KIT Technical data sheet.pdf

Download DENUDATION KIT Safety data sheet.pdf