MEDIUM 199

This complex medium was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells.

The media can be formulated either with Earle salts or Hanks Salts. The media formulated with the Earle salts are buffered with a bicarbonate / carbonic acid solution and retain their pH in a CO2 incubator. The use of the Earle salts under ambient conditions results in a rapid rise in the pH of the culture medium.

The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions and their use in a CO2 incubator results in a rapid drop in the pH of the culture medium.